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M94A3232.TXT
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1994-10-25
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Document 3232
DOCN M94A3232
TI Association between changes of HIV-1 quasispecies in serum and disease
progression.
DT 9412
AU Ida S; Oka S; Hirabayashi Y; Shioda T; Nagai Y; Kimura S; Shimada K;
Institute of Medical Science, University of Tokyo, Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):123 (abstract no. PA0112). Unique
Identifier : AIDSLINE ICA10/94369343
AB OBJECTIVE: In order to address the virulent variants which correlate
with clinical deterioration, we analyzed the genetic changes of HIV-1 in
sera obtained from three patients whose CD4 counts had declined rapidly.
MATERIALS AND METHODS: All patients are Japanese hemophiliacs who were
infected with HIV-1 at least before 1985. Rapid decline of CD4 counts
was noted from 1989 to 1990 in these patients. Sera were obtained at
three to four time points during, before and after CD4 depression. Total
RNA extracted from 100 microliters of serum was reverse transcripted and
amplified for envelope V3 region by the nested PCR. The amplified DNA
was cloned and al least 10 clones were sequenced. RESULTS AND
CONCLUSION: Fluctuations in quasispecies were observed in all cases
during study periods. Among them, newly occurring clones having amino
acid substitution from serine to arginine (positively charged amino
acid) at position 11 from the first cysteine of the V3 loop were
detected as a major clone in each patient four to six months before the
deterioration in all cases. These clones have evolved with additive
mutations thereafter. It might be postulated that these mutations lead
to not only antigenic variation to escape from the host immunity but
also adding virulency of the virus in vivo.
DE Acquired Immunodeficiency Syndrome/BLOOD/*MICROBIOLOGY/ PHYSIOPATHOLOGY
Hemophilia/COMPLICATIONS Human HIV-1/GENETICS/*ISOLATION &
PURIF/PATHOGENICITY *Point Mutation Polymerase Chain Reaction/METHODS
RNA, Viral/*BLOOD/ISOLATION & PURIF T4 Lymphocytes/IMMUNOLOGY Viral
Envelope Proteins/BIOSYNTHESIS Virulence MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).